QIAquick PCR Purification Kit Protocol using a microcentrifuge This protocol is designed to purify single- or double-stranded DNA fragments from PCR and other enzymatic reactions (see page 8). For cleanup of other enzymatic reactions, follow the protocol as described for PCR samples or use the MinElute Reaction Cleanup Kit. • QiaQuick PCR Purification kit (Qiagen, catalog # ) • Charge-Switch PCR Clean-up kit (Life Technologies, catalog # CS) • PureLink PCR Purification kit (Life Technologies, catalog # K) Other kits are acceptable if they produce purified PCR products suitable for sequencing. Please refer to the kit manual for instructions. The ChargeSwitch®-Pro Kit provides: • % ethanol-free, guanidinium-free, organic solvent-free protocols • Improved downstream performance • Complete kit for fast, hassle-free setup • 20% fewer handling steps than most PCR clean-up protocols • Purification of up to µl of PCR product in one column How It Works.
Description. PureLink Viral RNA/DNA Mini Kit provides rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants. Designed to isolate high-quality viral nucleic acids from variety of RNA and DNA viruses within 45 minutes. Offers ability to perform both a gel extraction or PCR purification with single kit Simple procedure uses silica-based spin cartridge to purify DNA fragments from 40bp to 10kb in PCR products, and salts from PCR fragments. The Wizard® SV Gel and PCR Clean-Up System (Promega; Pr), the GeneJET PCR Purification Kit (Thermo Fisher Scientific; Th), the PureLink® PCR Purification Kit (Invitrogen; In) and the Chromatin IP DNA Purification Kit (Active Motif; Am) performed poorly with de-crosslinked chromatin Consistently, these reagents recovered less than 50% of.
User Manual Corporate Headquarters Invitrogen Corporation Faraday Avenue Carlsbad, CA T: 1 7F: 1 7E: www.doorway.rue@www.doorway.ru For country-specific contact information visit our web site at www.doorway.ru PureLink™ HQ 96 Plasmid DNA Purification Kit For high-throughput isolation of high-quality. The Invitrogen™ PureLink™ Microbiome DNA Kit enables fast of high-quality microbial and host DNA from a wide variety of sample types. The kit uses proven PureLink™ spin-column technology for robust yields of DNA that is ready for downstream PCR, sequencing, or other applications. Typical DNA recovery is 5–20 µg from 1 mL of microbial. Expression Kit. For more information, refer to our website (www.doorway.ru) or call Technical Support (see page 78). Accessory Products Many of the reagents supplied in the EasySelect™ Pichia Expression Kit are available separately from Invitrogen. Ordering information is provided below. Product Reactions or Amount Cat. no.
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